Figure 7

The expression profile of Vv-AMP1 within the grapevine cultivar Pinotage. (A) Northern blot analysis of total RNA isolated from leaf (L) and flower tissue (F) as well as four berry developmental stages: Berry set (Bs), Véraison (Vb), Post-véraison (Pvb), Ripe (Rb). (B) Induction of Vv-AMP1 by developmental regulation (C). Biotic and abiotic induction studies were conducted on Pinotage leaf tissue. Time points indicate the time of tissue collection after the initiation of each induction experiment. Northern blot signals were detected by probing with a DIG labelled Vv-AMP1 cDNA and a V. vinifera EF-α1 probe as internal standard. The Vv-AMP1 signal hybridized at a molecular weight of 500 bp and the internal standard at 2500 bp.