Figure 8
From: Vv-AMP1, a ripening induced peptide from Vitis vinifera shows strong antifungal activity
Size determination and Western blot analysis of the purified recombinant Vv-AMP1 peptide. (A) SDS-PAGE analysis of the GST-fusion protein before and after thrombin treatment; lane M, low molecular weight marker (Sigma, St Louis, USA); lane 1 GST-fusion protein, lane 2 and 3 purified Vv-AMP1 peptide after thrombin digestion and cation exchange chromatography; lanes 4 and 5, Western blot analysis of Vv-AMP1. (B) Mass spectrometric analysis of recombinant Vv-AMP1 after separation from the GST-tag using ion exchange chromatography.