Figure 1

Time-lapse imaging of actin microfilaments and cell plate. (A) Time-lapse images of actin microfilaments and FM4-64-stained cell plates from metaphase to late telophase in a BY-GF11 cell. Green and red indicate GFP-ABD2 and FM4-64 fluorescence, respectively. Asterisks indicate actin microfilament twin peaks that appeared at metaphase [37]. We defined 0 min as the end of metaphase in this figure. Scale bar indicates 25 μm.(B) Changes in GFP-ABD2 and FM4-64 fluorescence intensities around the division plane. Intensities were time-sequentially measured along the division plane at a width of 9 μm, shown as the boxed region in (A). Data in (B) are mean values ± SE of four independent experiments. (C) Kymographs obtained by drawing a line across a cell plate from metaphase to late telophase, as shown by the yellow arrow in (A). Yellow broken lines indicate changes in MF localization during cell plate development. One representative experiment of four independent experiments is shown.