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Figure 2 | BMC Plant Biology

Figure 2

From: Quantitative analysis of changes in actin microfilament contribution to cell plate development in plant cytokinesis

Figure 2

Three-dimensional localization of actin microfilaments at early and late telophase. (A) Single optical section of a BY-GF11 cell at early telophase. Green and red indicate GFP-ABD2 and FM4-64 fluorescence, respectively. (B) Intensity profiles of GFP-ABD2 and FM4-64 along the line segment PQ in (A).(C) and (D) Cross-sections of GFP-ABD2 (C) and FM4-64 (D) along the line segment PQ in (A). (E) Merged images of (C) and (D). (F) Single optical section of a BY-GF11 cell at late-telophase. Green and red indicate GFP-ABD2 and FM4-64 fluorescence, respectively. (G) Intensity profiles of GFP-ABD2 and FM4-64 along the line segment RS in (F). (H) and (I) Cross sections of GFP-ABD2 (H) and FM4-64 (I) along the line segment RS in (F). (J) Merged images of (H) and (I). Optical sections, which were used for reconstruction of a cross-section, were taken at 0.5 μm intervals. Scale bars indicate 10 μm.

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