Figure 7

Effects of bistheonellide A (BA) on organization of phragmoplast-related endoplasmic reticulum (ER). (A) Time-lapse images of GFP-labeled endoplasmic reticulum (ER) and FM4-64-labeled cell plate during cytokinesis in a control cell. ER accumulated around the edge of the expanding cell plate before completion of cytokinesis (yellow arrowheads). (B) Projection image of ER at late telophase in a control cell. (C) Time-lapse images of GFP-labeled ER and FM4-64-labeled cell plate in a BA-pretreated cell. Accumulation of ER in the phragmoplast was inhibited (yellow arrows) compared with the control cell shown in (A). (D) Projection image of ER at late telophase in a BA-treated cell. (E) Changes in GFP and FM4-64 fluorescence intensities around the division plane. Intensities were time-sequentially measured in the division plane at a width of 9 μm, as shown in the boxed region of (A) and (C). Data in (E) are mean values ± SEs of four independent experiments.