Figure 7

The regulation of transcription of single-repeat R3 MYB genes by TTG1-GL3/EGL3-GL1 activator complex. (A) Expression of TTG1, GL1, WER, GL3 and EGL3 in seedlings and protoplasts. RNA was isolated from 10-day-old seedlings or from protoplasts isolated from the rosette leaves of 3-4-week-old plants. RT-PCR was use to examine the expression of TTG1, GL1, WER, GL3 and EGL3. (B) Regulation of single-repeat R3 MYB genes by GL1-GL3/EGL3 and WER-GL3/EGL3. Effector gene(s) were transfected or cotransfected into protoplasts. RNA was isolated from transfected protoplasts that had been incubated for 20–22 h in the darkness. RT-PCR was used to examine the expression of single-repeat R3 MYB genes. (C) Expression of single-repeat R3 MYB genes in gl3 egl3 double mutant. RNA was isolated from 10-day-old seedlings. RT-PCR was used to examine the expression of TRY, CPC, ETC1 and ETC3. The expression of ACTIN2 was used as control.