Modulation of AtTIL protein level affects development. (a) Genomic organization of Arabidopsis SALK lines carrying T-DNA insertions in the AtTIL gene [GenBank:BAB10998; Locus:At5g58070]. Boxes 1 and 2 are exons. The primers used for PCR analysis of the genomic DNA are indicated (see also Methods). (b) AtTIL protein levels in leaf extracts. Top panel: A rabbit anti-AtTIL antibody was used for immunoblot analysis. Bottom panel: Coomassie Brilliant Blue (CBB)-stained gel. (c) Plants were grown under normal conditions of temperature and photoperiod. Overhead (21 d) or lateral (28, 35, 45 d, 52 d) views are shown. (d) Developmental growth stages expressed according to Boyes et al. . 1.10: 10 rosette leaves; 5.10: appearance of the first flower buds; 6.00: opening of the first flower; 6.90: completion of flowering. At least 30 plants per line per assay were monitored, and the experiment was repeated 3 times. WT, wild type Col-0 plants; SALK_XXXXXX, AtTIL T-DNA insertion lines from the SALK collection; Comp, SALK_136775 KO plant complemented by overexpression of AtTIL; OEX, an AtTIL overexpressing line; Vector, Col-0 transformed with a binary vector that does not carry the AtTIL cDNA (negative control).