Accumulation of the AtTIL protein immunodetected with the anti-AtTIL antibody. (a) Proteins were purified from wild type plants grown under normal conditions, at various stages of their life cycle. (b) AtTIL protein levels in 7 day-old seedlings grown under light (L) or dark (D) conditions. Bottom panels: CBB-stained gel. WT, wild type Col-0 plants; KO, AtTIL T-DNA insertion lines from the SALK collection; Comp, KO plant complemented by overexpression of AtTIL; OEX, an AtTIL overexpressing line; Vector, Col-0 transformed with a binary vector that does not carry the AtTIL cDNA (negative control).