Figure 5

AtTIL knock-out plants show reduced hypocotyl elongation, hydrogen peroxide and other ROS accumulation. (a) Representative seedlings grown under dark conditions (upper panel), then stained with DAB (middle panel) to detect H₂O₂ and other ROS, or returned to light conditions for 24 h (lower panel). (b) Close-up of hypocotyls from dark-grown plants stained with DAB. (c) Representative seedlings grown under light-dark cycles (upper panel), then stained with DAB (lower panel). (d) Representative seedlings grown under continuous light (upper panel), then stained with DAB (middle panel). A close-up is shown to better show the DAB staining (lower panel). (e) Fluence response curves of seedlings grown under continuous light. Dark-adapted seedlings were exposed 7 days in the dark or under various intensities of continuous light. Images were captured and hypocotyl length was measured with the Image J software.