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Table 5 Previously characterised flavonoid biosynthesis pathway genes in wheat.

From: Relationship between homoeologous regulatory and structural genes in allopolyploid genome – A case study in bread wheat

  Gene cloning Mapping
Enzyme Cloning approach Number of cloned copies Genbank accessions; references Chromosome location; references
PAL – phenylalanine ammonialyase Isolation from genomic library 2 complete X99705[41, 42] 3A, 3B, 3D, 6A, 6B, 6D [7]
CHS – chalcone synthase PCR-based cloning 4 complete AY286093, AY286095, AY286096, AY286097[43] 1A, 1B, 1D, 2A, 2B, 2D [7]
CHI – chalcone-flavanone isomerase PCR-based cloning 1 partial AB187026[11] 5A, 5B, 5D [7]
F3H – flavanone 3-hydroxylase PCR-based cloning 1 partial AB187027[11] -
F3'5'H – flavonoid 3',5'-hydroxylase PCR-based cloning 1 partial AY519468[43] -
DFR – dihydroflavonol-4-reductase PCR-based cloning 3 complete AB162138, AB162139, AB162140[44] 3AL, 3BL, 3DL [44, 45]
ANS – anthocyanidin synthase Not described 5 complete AB247917, AB247918, AB247919, AB247920, AB247921[46] 6AS (2 copies), 6BS (2 copies), 6DS [46]
FMT – flavonoid 7-O-methyltransferase - - - 1A, 1B, 1D [7]