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Table 6 Primers designed to amplify wheat F3H for cloning, chromosomal localization and for expression analysis.

From: Relationship between homoeologous regulatory and structural genes in allopolyploid genome – A case study in bread wheat

Purpose

Gene

Gene segment specification (according Figure 1) or former gene name

DNA/cDNA-derived PCR product length (bp)

Forward primers

Reverse primers

PCR-based cloning

F3H

Segment 1

variable

atggcgccggtgagcaac

tttacgtggcatggcatgcat

 

F3H

Segment 2

variable

atgacgcgcctctctcgcg

tggacggtgatccaggtcttg

 

F3H

Segment 3

variable

[11]

[11]

 

F3H

Segment 4

variable

tctcgatcgatcgaccaccaa

ctaggcaagaatttcgttgaggg

 

F3H

Segment 5

variable

ccggtgagcaacgagacgttc

ggcaagaatttcgttgagggg

Chromosomal assignment and

F3H-A1

T. aestivum F3H1

703/-

gccacctgcaggtatacacgcat

ccaccgcccgtagtccct

physical mapping

F3H-B1

T. aestivum F3H3

333/-

gcgtgctgtccgaggcgc

cgatcgatcgattaaggatt

 

F3H-B2

T. aestivum F3H4

255/155

gctgcctgccgaggacaagg

aacgcccgtagtcccgtgcc

 

F3H-D1

T. aestivum F3H2

225/-

gccacctgcaggtacccacacat

ccacctcccgtagtcccg

 

F3H-G1

T. timopheevii F3H2 t

371/-

acgactcatggggctgtca

caattggtggtcgatcgatcag

qRT-PCR, RT-PCR

F3H-A1

T. aestivum F3H1

800/186

atgacacgcctctctcgcg

ccaccgcccgtagtccct

 

F3H-B1

T. aestivum F3H3

830/183

tgacgcgcctctctcgcgag

accgcccgtagtcccgtgct

 

F3H-B2

T. aestivum F3H4

255/155

gctgcctgccgaggacaagg

aacgcccgtagtcccgtgcc

 

F3H-D1

T. aestivum F3H2

281/145

atcgtctccagccacctgcag

cgctgtatcgctccaccacg