Table 3 Real-time PCR validation of microarray results.
From: Physiological and molecular characterization of aluminum resistance in Medicago truncatula
| Â | Â | Â | Â | Â | qPCR fold change | Ratio (T32/S70) | ||
|---|---|---|---|---|---|---|---|---|
TIGR TC | Annotation | Time (h) | M. truncatula line | Microarray fold change | Mean | SE | Array | qPCR |
TC105342 | MATE | 12 | T32 | 39.8 | 44.0 | 7.8 | 0.8 | 0.7 |
| Â | Â | Â | S70 | 51.3 | 67.4 | 6.4 | Â | Â |
TC95697 | F-box protein | 12 | T32 | 5.0 | 13.7 | 4.0 | 3.6 | 4.4 |
| Â | Â | Â | S70 | 1.4 | 3.1 | 0.2 | Â | Â |
TC103586 | ZPT-2 | 12 | T32 | 2.9 | 2.4 | 0.2 | 0.8 | 0.4 |
| Â | Â | Â | S70 | 3.5 | 6.1 | 0.9 | Â | Â |
TC111698 | COBRA-like gene | 12 | T32 | 16.3 | 24.7 | 0.6 | 0.9 | 0.5 |
| Â | Â | Â | S70 | 18.8 | 53.7 | 16.4 | Â | Â |
TC93997 | PR-protein PR-4 | 12 | T32 | 2.6 | 2.5 | 0.3 | 0.5 | 0.5 |
| Â | Â | Â | S70 | 5.0 | 5.0 | 1.1 | Â | Â |
TC100486 | Xyloglucan endotransglycosylase | 12 | T32 | 2.3 | 2.2 | 0.1 | 1.8 | 1.6 |
| Â | Â | Â | S70 | 1.3 | 1.4 | 0.3 | Â | Â |
TC96658 | Xyloglucan endotransglycosylase | 48 | T32 | 2.9 | 3.7 | 1.0 | 0.3 | 0.1 |
| Â | Â | Â | S70 | 10.7 | 38.3 | 5.6 | Â | Â |
TC102211 | GAST-1 | 48 | T32 | 2.1 | 7.2 | 0.4 | 1.6 | 0.3 |
| Â | Â | Â | S70 | 1.3 | 25.8 | 5.7 | Â | Â |
TC111920 | Polygalacturonase PG1 putative | 48 | T32 | 2.8 | 2.5 | 0.2 | 0.8 | 0.6 |
| Â | Â | Â | S70 | 3.7 | 4.0 | 0.5 | Â | Â |
TC103771 | Ethylene up- regulated gene ER66 | 48 | T32 | 1.2 | 1.3 | 0.2 | 0.5 | 0.3 |
| Â | Â | Â | S70 | 2.3 | 4.6 | 0.8 | Â | Â |
TC95154 | Peroxidase | 48 | T32 | 3.9 | 1.2 | 0.1 | 1.3 | 0.4 |
| Â | Â | Â | S70 | 3.0 | 2.9 | 0.1 | Â | Â |
TC100155 | Lipoxygenase | 48 | T32 | 0.5 | 0.4 | 0.0 | 0.7 | 0.6 |
| Â | Â | Â | S70 | 0.7 | 0.6 | 0.1 | Â | Â |