Figure 5

Sequence similarity and genomic organization of VicTR-A subfamilies in Vicia pannonica. (A) Alignment of the clones c666 and c653, representing the subfamilies with 69/138 and 180 bp periodicities. The A-tracts involved in recombination between the 69/138 repeats which presumably gave rise to the 180 bp subfamily are indicated with orange lines, while other A-tracts are marked with black lines. Only a part of the alignment is shown. (B) Dot-plot comparison of the clones c666 and c653, displaying similarities between the sequences as diagonal lines [48]. (C) Southern blot of V. pannonica genomic DNA digested with various enzymes and hybridized with probes derived from VicTR-A clones c666 and c653, respectively. Enzymes used for DNA digestion were: S, Sau3A; M, MboI; T, TaqI; B, BsmFI; N, NlaIII; Bp, Np, partial restriction digestion of BsmFI and NlaIII, respectively.