Figure 5

CR DNA methylation in the Bor-4 accession. (A) Increased NOR DNA methylation in Bor-4 plants transformed with a VIM1 transgene under the control of the 35S promoter. Genomic DNA samples of the indicated genotypes were digested with HpaII and used for DNA gel blot analysis with a hybridization probe for the CR region. (B) Plot of CR DNA methylation index versus NOR and VIM1 genotypes in a Bor-4 × Col F2 population. The F2 individuals were classified into nine groups based on their genotypes at NOR2-NOR4 (C = Col/Col, H = Col/Bor-4, R = Bor-4/Bor-4), and then each group was divided into two groups based on the presence of the vim1-1 mutation as a homozygote. Vertical bars denote standard errors. The values obtained for the parental accessions are indicated by red horizontal lines.