Figure 2
Analysis of proteins extracted by CaCl 2 and LiCl from the cell wall fraction prepared from 11 day-old hypocotyls. a. Separation of proteins by cation exchange chromatography. The graph shows amounts of proteins in each fraction eluted by a NaCl gradient (from 0 M to 0.8 M) followed by two steps at 1.2 M and 1.5 M NaCl. Dotted vertical lines show the grouping of chromatography fractions. Ub stands for unfixed fraction, Wa for washes of the column prior to protein elution, numbering to FPLC fractions, and letters (from N to Z) to pools analyzed by 1D-E. b. Separation by 1D-E of the total protein extract (total) and of fractions N to Z obtained after cation exchange chromatography. Molecular mass markers are on the right. Numbers refer to bands analyzed by MALDI-TOF MS with successful identification (see Additional data file 2).