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Table 2 CWPs identified in salt extracts of cell wall fractions prepared from 5 and 11 day-old etiolated hypocotyls of Arabidopsis

From: A new picture of cell wall protein dynamics in elongating cells of Arabidopsis thaliana: Confirmed actors and newcomers

accession

annotation

5 days

11 days

11 days vs5 days

AGI

    

proteins acting on carbohydrates

At2g06850

GH family 16 (AtXTH4)

+

+

<

At5g13870

GH family 16 (AtXTH5)

+

-

 

At3g44990

GH family 16 (AtXTH31)

+

-

 

At2g36870

GH family 16 (AtXTH32)

-

+

 

At1g10550

GH family 16 (AtXTH33)

+

+

<

At4g16260

GH family 17

-

+

 

At4g19810

GH family 18 (chitinase)

+

-

 

At3g12500

GH family 19 (chitinase)

+

+

At3g55260

GH family 20 (beta-hexosaminidase)

+

-

 

At1g19170

GH family 28 (polygalacturonase)

+

-

 

At2g33160

GH family 28 (polygalacturonase)

+

-

 

At3g06770

GH family 28 (polygalacturonase)

-

+

 

At3g16850

GH family 28 (polygalacturonase)

+

+

At3g61490

GH family 28 (polygalacturonase)

+

+

At4g18180

GH family 28 (polygalacturonase)

+

-

 

At1g68560

GH family 31 (alpha-xylosidase) (AtXYL1)

+

+

At3g13790

GH family 32 (beta-fructofuranosidase)

+

+

At5g34940

GH family 79 (endo beta-glucuronidase/heparanase)

+

+

<

At1g11580

CE family 8 (pectin methylesterase)

+

-

 

At1g53830

CE family 8 (pectin methylesterase) (AtPME2)

+

+

<

At3g14310

CE family 8 (pectin methylesterase) (AtPME3)

+

+

At3g43270

CE family 8 (pectin methylesterase)

+

+

At4g33220

CE family 8 (pectin methylesterase)

+

+

At5g53370

CE family 8 (pectin methylesterase)

+

-

 

At4g37950

PL family 4 (rhamnogalacturonate lyase)

-

+

 

At1g05570

GT family 48 (callose synthase) (AtCalS1)

+

+

At5g02260

alpha-expansin (AtEXPA9)

+

+

<

At1g20190

alpha-expansin (AtEXPA11)

+

+

At5g39270

alpha expansin (AtEXPA22)

+

-

 

At3g45970

expansin-like A (AtEXLA1)

+

+

At4g38400

expansin-like A (AtEXLA2)

+

+

<

At3g45960

expansin-like A (AtEXLA3)

+

-

 

oxido-reductases

At1g71695

peroxidase (AtPrx12)

+

+

>

At3g21770

peroxidase (AtPrx30)

+

+

At3g32980

peroxidase (AtPrx32)

+

+

<

At3g49110

peroxidase (AtPrx33)

-

+

 

At3g49120

peroxidase (AtPrx34)

+

+

At3g50990

peroxidase (AtPrx36)

+

-

 

At4g25980

peroxidase (AtPrx43)

-

+

 

At4g30170

peroxidase (AtPrx45)

+

+

At5g17820

peroxidase (AtPrx57)

+

-

 

At5g64100

peroxidase (AtPrx69)

+

+

At5g66390

peroxidase (AtPrx72)

-

+

 

At2g30210

laccase homologue (AtLAC3)

+

+

At1g30710

berberine-bridge enzyme homologue

+

-

 

At4g20860

berberine-bridge enzyme homologue

-

+

 

At5g44360

berberine-bridge enzyme homologue

+

+

>

At5g44410

berberine-bridge enzyme homologue

-

+

 

At1g01980

berberine-bridge enzyme homologue

-

+

 

At2g02850

plantacyanin ARPN (blue copper binding protein)

-

+

 

At4g12880

early nodulin AtEN20 (blue copper binding protein)

+

+

<

At5g22140

expressed protein (oxido-reductase domain)

+

-

 

At5g56490

expressed protein (FAD binding domain)

+

-

 

proteins with interacting domains

At1g53070

lectin homologue (legume lectin domains)

+

+

<

At1g78820

lectin homologue (curculin-like)

+

-

 

At1g78830

lectin homologue (curculin-like)

+

+

At1g78850

lectin homologue (curculin-like)

+

+

At1g78860

lectin homologue (curculin-like)

+

+

<

At5g06860

PGIP1 (LRR domains)

+

+

T23B7.10

PGIP1 homologue (LRR protein FLR1)

+

+

>

At5g12940

Phaseolus vulgaris PGIP2 homologue (LRR domains)

+

+

At1g33590

expressed protein (LRR domains)

+

+

At2g34930

expressed protein (LRR domains)

+

+

At3g20820

expressed protein (LRR domains)

+

+

>

At2g17120

expressed protein (LysM domain)

+

+

<

At1g03220

carrot EDGP and tomato XEGIP homologue

+

+

At1g03230

carrot EDGP and tomato XEGIP homologue

+

+

At5g19110

carrot EDGP and tomato XEGIP homologue

+

+

<

At1g47710

serpin homologue (serine protease inhibitor)

+

+

<

At1g17860

inhibitor family I3 (Kunitz-P family)

+

+

At1g73260

inhibitor family I3 (Kunitz-P family)

+

+

>

At1g47540

inhibitor family I18 (mustard trypsin inhibitor-2 family)

+

+

nd

At2g40880

inhibitor family I25 (phytostatin)

+

+

At5g05110

inhibitor family I25 (phytostatin)

+

+

At4g16500

inhibitor family I25 (cystatin family)

+

+

>

At4g25260

invertase/pectin methylesterase inhibitor homologue

+

+

<

At5g46940

invertase/pectin methylesterase inhibitor homologue

+

-

 

At5g46960

invertase/pectin methylesterase inhibitor homologue

+

-

 

Proteases

At1g09750

aspartic protease homologue (pepsin family)

+

+

<

At3g02740

aspartic protease homologue (pepsin family)

+

-

 

At3g52500

aspartic protease homologue (pepsin family)

+

-

 

At3g54400

aspartic protease homologue (pepsin family)

+

+

At5g07030

aspartic protease homologue (pepsin family)

+

+

At1g79720

aspartic protease homologue (CND41 peptidase)

+

-

 

At5g10770

aspartic protease (CND41 peptidase)

+

+

At1g47128

cysteine proteinase homologue (papain family)

+

+

At5g43060

cysteine proteinase homologue (papain family)

+

+

At4g01610

cysteine proteinase homologue (papain family)

+

+

At4g36880

cysteine proteinase homologue (papain family)

+

-

 

At3g02110

serine carboxypeptidase D (SCPL25)

+

+

<

At5g23210

serine carboxypeptidase (SCPL34)

+

+

nd

At4g30610

carboxypeptidase homologue (BRS1 – Brassinosteroid-Insensitive BRI suppressor 1)

+

-

 

structural proteins

At1g28290

proline-rich protein

+

+

At5g14920

proline-rich protein

+

+

At2g05580

glycine-rich protein

+

+

>

At4g13340

LRR-extensin (AtLRX3)

+

-

 

At3g24480

LRR-extensin (AtLRX4)

+

+

>

At4g18670

LRR-extensin (AtLRX5)

+

+

signaling

At4g05200

receptor kinase homologue (RLK, DUF26-1b subfamily)

+

-

 

At5g55730

fasciclin-like arabinogalactan protein (AtFLA1)

+

+

>

proteins related to lipid metabolism

At1g29670

lipase acylhydrolase homologue (GDSL family)

+

+

<

At1g54010

lipase/acylhydrolase homologue (GDSL family)

+

-

 

At1g54030

lipase/acylhydrolase homologue (GDSL family)

+

+

At3g48460

lipase/acylhydrolase homologue (GDSL family)

+

+

<

At5g15720

lipase/acylhydrolase homologue (GDSL family)

-

+

 

At2g38530

non-specific lipid transfer protein type 1 (LTP2)

+

+

At5g23820

expressed protein (ML domain – MD-2-related lipid recognition domain)

+

-

 

At2g16001

expressed protein (lipid recognition domain)

+

-

 

miscellaneous functions

At2g27190

purple acid phosphatase homologue (PAP1)

+

+

<

At3g07130

purple acid phosphatase homologue

+

+

<

At5g34850

purple acid phosphatase homologue

+

-

 

At4g29270

acid phosphatase homologue

+

+

<

At4g24340

phosphorylase homologue homologue

+

-

 

At3g02870

myo-inositol monophosphatase homologue

+

-

 

At5g09440

Nicotiana tabacum phi-I homologue

+

+

At5g64260

Nicotiana tabacum phi-I homologue

+

+

At5g66590

Nicotiana tabacum pathogenesis-related protein PR1 homologue

+

+

At2g28790

Lycopersicon esculentum osmotin homologue

+

+

At5g15230

gibberellin-regulated protein (GASA4)

+

-

 

At4g27110

homologous to COBRA (AtCOBL10)

+

-

 

At1g09560

germin (subfamily 2, member 1, GLP5)

-

+

 

unknown function

At3g56750

expressed protein

-

+

 

At3g22000

expressed protein (DUF26)

-

+

 

At1g26850

expressed protein (DUF248)

-

+

 

At1g80240

expressed protein (DUF642)

+

+

nd

At3g08030

expressed protein (DUF642)

+

+

At4g32460

expressed protein (DUF642)

+

+

<

At5g11420

expressed protein (DUF642)

+

+

At5g25460

expressed protein (DUF642)

+

+

At1g78460

expressed protein (SOUL heme binding domain)

-

+

 

At2g04690

expressed protein (homologous to a human brain CREG protein)

+

+

At2g15220

expressed protein (Plant Basic Secreted Protein domain)

+

+

>

At2g34700

expressed protein (Ole e1 allergen domain)

+

+

At3g20370

expressed protein (MATH domain)

+

-

 

At2g28490

expressed protein (cupin domain)

+

+

<

At3g22640

expressed protein (cupin domain)

+

+

At4g36700

expressed protein (cupin domain)

+

-

 
  1. Accession numbers of genes encoding proteins predicted to have a GPI anchor or trans-membrane domains are in bold or in grey boxes respectively. Accession numbers of genes encoding proteins identified for the first time by using cell wall proteomics are underlined. Details of functional annotation are in Additional data files 1 and 2. + indicates the presence of proteins in 5 or 11 days-old hypocotyls. - means that the proteins were not identified. The relative amount of proteins in these two physiological stages is indicated in the right column (see Additional data file 6). nd means not determined.