Figure 8

Amylopectin chain length distribution. A, Chain length distributions. The population distribution of chains of a given DP was normalized to the total number of chains in the DP range of 5–55. For wild type and Atss2-1 the values shown are the mean from at least three independent biological replicates, with error bars indicating the standard error. Starch from the Atss2, Atss3 double mutant was analyzed only once owing to sample limitation. B, Difference plot comparison of chain length distributions. For each given DP, the distribution mean value for wild type was subtracted from that of the indicated mutant strain. The chain length distribution means used to generate the difference plots for the Atss2-2 and Atss2-3 lines are taken from data not shown. Appropriate wild type controls were used, i.e., Columbia for the Atss2-1 and Atss2-2 mutants, and WS for the Atss2-3 mutant. For comparison, the difference plot for the Atss3-1 line is shown from previously published results [29]. C, Pleiotropic effect plot. The predicted sum of the Atss2-1 and Atss3-1 effects in single mutants (solid line) is compared to the observed result for the double mutant (dotted line).