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Figure 1 | BMC Plant Biology

Figure 1

From: Cloning, characterisation and comparative analysis of a starch synthase IV gene in wheat: functional and evolutionary implications

Figure 1

A schematic diagram showing the PCR fragments that make up the contiguous SSIV cDNA as well as domains found within the predicted amino acid sequence. PCR fragments A, B and C were amplified by a 5'RACE-like procedure carried out with wheat cDNA libraries (see Methods) and represent the minimum tiling path of several sequences identified. The SSIV EST (EST-968 bp) and the cDNAs called 1600 bp RACE and 2100 bp RACE were isolated similarly to A, B and C and were assembled into different cloning vectors to test coding potential and/or glycosyltransferase activity. The starch catalytic domain (GT-5) and glycosyltranferase domain (GT-1) characteristics of the SS family are shown. Predicted 14-3-3 recognition sites and the coiled-coil domains (blue boxes and 'CC'), as well as the two highly conserved KVGGL and KTGGL domains are also shown.

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