Figure 1From: Cloning, characterisation and comparative analysis of a starch synthase IV gene in wheat: functional and evolutionary implicationsA schematic diagram showing the PCR fragments that make up the contiguous SSIV cDNA as well as domains found within the predicted amino acid sequence. PCR fragments A, B and C were amplified by a 5'RACE-like procedure carried out with wheat cDNA libraries (see Methods) and represent the minimum tiling path of several sequences identified. The SSIV EST (EST-968 bp) and the cDNAs called 1600 bp RACE and 2100 bp RACE were isolated similarly to A, B and C and were assembled into different cloning vectors to test coding potential and/or glycosyltransferase activity. The starch catalytic domain (GT-5) and glycosyltranferase domain (GT-1) characteristics of the SS family are shown. Predicted 14-3-3 recognition sites and the coiled-coil domains (blue boxes and 'CC'), as well as the two highly conserved KVGGL and KTGGL domains are also shown.Back to article page