Figure 2

Phylogenetic tree showing the relationship between plant and algal SS and prokaryotic GS isoforms on the basis of predicted amino acid sequence. The tree was constructed using the neighbour-joining method and confidence limits to branch points in the tree were assigned by bootstrapping the alignment with a random number generator seed of 111 and 1000 trials. Unicellular algae SSs are shown in blue and GSs are in red. Each node is labeled with the prefix of the initials of the genus and species, e.g. At = Arabidopsis thaliana Agt = Agrobacterium tumefascien; Cr = Chlamydomonas reinhardti; Sp = Synechocystis; Os = Oryza sativa;, Ot = Ostreococcus tauri; St = Solanum tubersum; Ta = Triticum aestivum; and Vu = Vignia unguiculata; Genbank accession numbers of the starch synthases and glycogen synthases shown in the tree are as follows: AtSSI [AAF24126], AtSSII [AAF26156]AtSSIII [AAD30251], AtSSIV [CAA16796], AtGBSSI [NP_174566], CrGBSSI [AAL28128], CrSSII [AAC17970], CrSSIII [AAY42381], CrSSIV [AAC17971], AgtGS [AAD03474], OsGBSSI [AAC61675], OsSSI [AAL16661], OsSSIIa [Q5DWW9], OsSSIIb [AAK81729], OsSSIIc [AAK64284], OsSSIIIa [AAM49811], OsSSIIIb [AAL40942], OsSSIVa [AAQ82622], OsSSIVb [AAQ82623], OsGBSSI [AAF72562], OsGBSSIb (called GBSSII [BAF26592]) SpGS-1 [P74521], SpGS-2 [P72623], StSSI [P93568], StSSIII [T07663], TaGBSSI [BAA77351], TaSSI [CAB99209], TaSSIIa [CAB86618], TaSSIIIa [AAF87999], TaSSIIIb [ABY56823]TaSSIV [AAK97773], VuSSIII [CAB40374], VuSSV [CAB40375], TaGBSSII [AAF14233], OtSSI [CAL56451], OtSSII [CAL58277], OtSSIIIa [AAS88893], OtSSIIIb [AAS88894] and OtSSIIIc [AAS88881].