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Figure 3 | BMC Plant Biology

Figure 3

From: Cloning, characterisation and comparative analysis of a starch synthase IV gene in wheat: functional and evolutionary implications

Figure 3

Partial genomic sequence of TaSSIVb showing the exon-intron arrangement. The TaSSIVb genomic clone was isolated by PCR using genomic DNA from a Triticum aestivum cv. Chinese Spring line nullisomic for chromosome 1B and tetrasomic for chromosome 1D as template. The primers used for amplification are indicated in the box and were designed from the cDNA sequence. The longest PCR fragments (A, B and C) that gave the minimum tiling path are indicated. Exon-intron organisation of the assembled genomic sequence was predicted by Spidey [75], which compares cDNA against genomic DNA sequence. Exons are indicated by the black boxes.

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