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Figure 4 | BMC Plant Biology

Figure 4

From: Cloning, characterisation and comparative analysis of a starch synthase IV gene in wheat: functional and evolutionary implications

Figure 4

Chromosomal Mapping of TaSSIVb by RFLP analysis of Chinese Spring ditelosomic lines. Approximately 20 μg of wheat genomic DNA was digested with EcoRV and blotted onto HyBond N+, and was hybridised against a 550 bp fragment from a PstI/EcoRI digest of clone 1600 bp RACE (Figure 1). The estimated size of each band is indicated on the right. The nomenclature for each sample is as follows: CS, Chinese Spring parental; Ditelosomic lines, 1AL, 1BL and 1DL, lack the short arms of chromosomes 1A, 1B and 1D, respectively, but each has four copies of the corresponding long arms. Ditelosomic lines, 1AS, 1BS and 1DS, lack the long arms of chromosome 1A, 1B and 1D, respectively, but each has four copies of the corresponding short arm. Bands missing in 1AS, 1BS and 1DS show that SSIV is likely located on the long arm of Chromosome I.

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