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Table 2 Amino acid residues within the substrate binding/catalytic sites in SSs that vary from the corresponding residue in AgtGS

From: Cloning, characterisation and comparative analysis of a starch synthase IV gene in wheat: functional and evolutionary implications

 

SSs: Group A

 

SSs: Group B

AgtGS

GBSSI

SSI

SSII

SSIII

SSIV

Thr16

Thr92

Ser/Thr154

Thr323

Val1195

Val436

Gln140

His231

His287

His449*

Ser1311â‘ 

Gln560

Ile162

Ile257

Ile313

Ile475

Ile1336

Cys585â‘¡

Phe167

Tyr/Phe262

His-318

His480

Tyr/Phe1341

Tyr590â‘¡

Ile297

Val/Ile400

Ile460

Ile619

Val/Ile1437

Val/Ile724

Ser298

Gly401

Gly461

Gly620

Ser/Thr1438

Ser/Thr725

Gly329

Gly433

Gly492

Gly651

Ala1469

Ser756â‘¡

Gly353

Arg/Lys457

Gly516

Gly675

Arg/Lys/Tyr/Thr1498

Lys783â‘¡

Asn355

Ser/Asn459

Ser/Asn518

Ser677

Asp1500

Asp785â‘¡

Ser359

Ala463

Ala/Ser522

Ala681

Ser1504

Ser789â‘¡

Thr381

Ile485

Asn554

Asn703

Ser/Thr1526

Thr811

  1. Amino acids identified in AgtGS as part of the ADPglucose pocket and are involved in binding and/or catalysis that were not conserved across all of the SS isoforms are shown. Non-conservative amino acids changes that could affect enzyme properties are emboldened. Data was extracted from the alignment shown in Figure 8. Species with an amino acid at the residue other than that in the table are indicated as follows: ①OtSSIIIa/c where the residue is a glutamine. ②CrSSIV. *OsSSIIa – Tyr