Figure 4

RT-PCR analysis to detect transcripts of thirteen M. truncatula genes using RNA from mock-inoculated roots, and from mycorrhizal roots. RNA from root pieces from M. truncatula mock-inoculated roots (Mt3) or M. truncatula/G. versiforme mycorrhizal roots (Mt/Gv3), and from M. truncatula mock-inoculated roots (Mt4) or M. truncatula/G. intraradices mycorrhizal roots (Mt/Gi4) was used. The M. truncatula transgenic plant line pMtSCP1::GFP was used and sampling of colonized root pieces was guided by the expression of GFP. Genes selected for analysis were up-regulated ≥2-fold in the Medicago GeneChip^® experiment. *TC104979 was reported previously as mycorrhiza-induced. MtPT4 was used as positive control and elongation factor 1α (TC106485) was included as endogenous control.