Figure 3

Unrooted phylogenetic tree of the GTases based on amino acid sequence similarity. GenBank accession numbers and sources for the respective protein sequences are: CsGT45 (FJ194947) from Crocus sativus; flavonoid 3-O-glucosyltransferases from Arabidopsis thaliana (AAD17392), AtUGT73B4 and (At5G17050), At GT; Zea mays (X13502), ZmF3GT; from Vitis vinifera (AAB81682), VvF3GT; from Fragaria × ananassa (BAA12737), GtF3GT; from Dianthus caryophyllus (BAD52005), DicGT3 and (BAD52003), DicGT1; At3RhaT, flavonol 3-O-rhamnosyltransferase from Arabidopsis thaliana (At1g30530); At7RhaT, flavonol 7-O-rhamnosyltransferase from Arabidopsis thaliana (NP_563756); flavonoid 7-O-glucosyltransferases from Scutellaria baicalensis (BAA83484), ScbF7GT; Pyrus communis (AAY27090), PcF7GT; from Nicotiana tabacum (BAB88935), NtF7GT from Arabidopsis thaliana (AAR01231), AtF7GT; NtSalGT, salicylic acid glucosyltransferase from Nicotiana tabacum (AAF61647); AtUGT73B3, pathogen-responsive glucosyltransferase from Arabidopsis thaliana (AAD17393); DicGT4, chalcononaringenin 2'-O-glucosyltransferase (BAD52006) from Dianthus caryophyllus; DbBet5GT, betanidin-5-O-glucosyltransferase from Dorotheanthus bellidiformis (CAB56231); UGT74F1, UGT74F2, and UGT73B1, flavonoid glucosyltransferases from Arabidopsis thaliana (AAB64022.1), (AAB64024.1) and (At4g34138); Letwi1, wound-inducible glucosyltransferase from Solanum lycopersicum (CAA59450); NtIS5a, immediate-early salicylate-induced glucosyltransferase from Nicotiana tabacum (AAB36653); FaGT7, multi-substrate flavonol-O-glucosyltransferase (ABB92749); AtF3GTb, putative flavonol 3-O-glucosyltransferases from Arabidopsis thaliana (NP_180535.1), AtF3GTc and (NP_180534.1), from Petunia hybrida (AAD55985), PhF3GT; from Gentiana triflora (BAA12737), GtF3GT; from Dianthus caryophyllus (BAD52004), DicF3GT; DbBET6GT, betanidin 6-O-glucosyltransferase from Dorotheanthus bellidiformis (AAL57240); UGT71B6, glucosyltransferase from Arabidopsis thaliana (AB025634); FaGT3 and FaGT7, flavonol-O-glucosyltransferases from Fragaria × ananassa (AAU09444) and (ABB92748); NtGT1a and NtGT1b, broad substrate specificity glucosyltransferases from Nicotiana tabacum (BAB60720) and (BAB60721); AtA5GT, glucosyltransferase from Arabidopsis thaliana (AAM91686); anthocyanin 5-O-glucosyltransferases from Torenia hybrida (BAC54093), ThA5GT; from Verbena hybrida (BAA36423), VhA5GT; from Perilla frutescens (BAA36421), PfA5GT; from Petunia hybrida (BAA89009), PhA5Gt; UGT71F1, regioselective 3,7 flavonoid glucosyltransferase from Beta vulgaris (AY526081); UGT73A4, regioselective 4',7 flavonoid glucosyltransferases from Beta vulgaris (AY526080); UGT71G1, triterpene glucosyltransferase from Medicago truncatula (AAW56092). The horizontal scale shows the number of differences per 100 residues derived from the ClustalW alignment.