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Table 1 The kinetic parameters Km and Vmax and the relation (Vmax/Km) of CsGT45, toward kaempferol and quercetin with a fixed UDPG concentration.

From: Cloning and characterization of a glucosyltransferase from Crocus sativusstigmas involved in flavonoid glucosylation

substrate

Km (μM)

Vmax (pkat/mg protein)

Vmax/Km

Kaempferol 7-OH

15.6 ± 1.2

366 ± 19.8

23.46

Quercetin 4'-OH

86.95 ± 8.4

186 ± 12.4

2.14

Quercetin 3'-OH

30.3 ± 3.6

22.9 ± 2.6

0.75

Quercetin 7-OH

21.50 ± 2.3

104 ± 5.46

4.83

  1. Enzyme assays were carried out using purified CsGT45 (7 μg), substrate (20 to 100 μM) and UDP-glucose (2.5 mM). Reactions mixtures were incubated at 30°C, and performed in triplicate.
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BMC Plant Biology

ISSN: 1471-2229

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