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Table 3 List of the spots identified in the leaves and their change in abundance after the exposure to 10 mM nitrate for 30 h.

From: Evaluation of protein pattern changes in roots and leaves of Zea maysplants in response to nitrate availability by two-dimensional gel electrophoresis analysis

Spot ID

Accession number

Protein description

Abbr.a

M r b/pIb

M r c/pIc

Change in level

[Relative volume (%)]

      

Control

10 mM NO3

Nitrogen and amino acid metabolism

1094

BAB11740

TaWIN2

TaWIN2

29.9/4.7

28.7/4.8

0.182 ± 0.009

0.090 ± 0.014

254

AAL73979

Methionine synthase protein

MetS

83.4/5.9

83.8/5.9

0.148 ± 0.020

0.073 ± 0.008

C-compound and carbohydrate metabolism

650

AAC27703

Putative cytosolic 6-phosphogluconate dehydrogenase

6PGD

47.4/6.0

52.9/6.2

0.088 ± 0.005

0.043 ± 0.007

Photosynthesis

134

P04711

Phosphoenolpyruvate carboxylase 1

PEPCase-a

104.4/5.8

109.3/5.8

0.990 ± 0.083

2.770 ± 0.295

138

P04711

Phosphoenolpyruvate carboxylase 1

PEPCase-b

104.4/5.7

109.3/5.8

2.220 ± 0.278

1.090 ± 0.205

500

P05022

ATP synthase subunit alpha, chloroplastic

ATPsyn α

55.9/6.1

55.7/5.9

0.042 ± 0.007

0.015 ± 0.003

1065

NP_001063777

Putative triosephosphate isomerase, chloroplast precursor d

TIM

31.0/4.9

32.4/7.0

0.028 ± 0.009

0.088 ± 0.014

1244

Q00434

Oxygen-evolving enhancer protein 2, chloroplast precursor

OEE2

26.6/6.5

27.3/8.8

0.201 ± 0.013

0.090 ± 0.011

1612

BAA08564

23 kDa polypeptide of photosystem II

23pPSII

26.3/6.5

27.0/9.5

0.147 ± 0.008

0.055 ± 0.006

Protein folding and stabilization

462

NP_001056601

RuBisCO subunit binding-protein beta subunit d

CPN-60 β

58.5/5.1

64.1/5.6

0.079 ± 0.014

0.164 ± 0.015

467

AAP44754

Putative rubisco subunit binding-protein alpha subunit precursor

CPN-60 α

58.2/4.8

61.4/5.4

0.046 ± 0.004

0.096 ± 0.004

Metabolism of vitamins, cofactors, and prosthetic groups

999

Q41738

Thiazole biosynthetic enzyme 1-1, chloroplast precursor

TH1-1

33.0/5.1

32.8/4.9 e

0.010 ± 0.001

0.048 ± 0.003

Secondary metabolism

313

AAL40137

Phenylalanine ammonia-lyase

PAL

70.2/6.0

74.9/6.5

0.076 ± 0.008

0.023 ± 0.002

Lipid metabolism

219

ABC59693

Lipoxygenase

LOX

94.6/5.8

102.1/6.1

0.023 ± 0.011

0.149 ± 0.011

  1. Statistical information about LC-ESI-MS/MS analysis are reported in Additional files 2 and 3. Changes in the relative spot volumes are the mean ± SE of six 2-DE gels derived from three independent biological samples analyzed in duplicate (n = 6). Proteins were classified according to MIPS funcat categories.
  2. a: Protein abbreviation
  3. b: Experimental molecular weight (kDa) or isoelectric point
  4. c: Theoretical molecular weight (kDa) or isoelectric point
  5. d: Information obtained by alignment of the sequence through BLAST analysis against NCBI nr database
  6. e: Values referred to the mature form of the protein