Figure 3

Distribution of mutations detected by the polyacrylamide/ethidium bromide platform within the target sequence. The position of each confirmed mutation (N = 141) in the seven targeted gene/genome combinations in hexaploid wheat is plotted against the target sequence scaled to 100%, with each bin representing 5% of the target sequence. No mutations were detected in the first and last two bins (0–10% and 90–100%) which represent the sequence closest to either forward or reverse primers.