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Table 1 Characteristics of TILLING targets and mutation frequencies in the hexaploid and tetraploid TILLING populations

From: A modified TILLING approach to detect induced mutations in tetraploid and hexaploid wheat

Gene

Pop.

Chr.

Size (bp)

GC content (%)

M2plants screened

Mutations

Mutation Frequency

WKS1

6×

6B

1371

39.8

1536

28

1/60 kb

 

6×

6B

1270

40.7

1536

43

1/37 kba

WKS2

6×

6B

1460

39.1

768

25

1/36 kb

 

6×

6B

1532

39.8

768

25

1/42 kbb

SBEIIa

6×

2A

1593

37.7

1536

40

1/49 kb

 

6×

2B

1638

37.7

768

17

1/59 kb

 

6×

2D

1614

37.2

768

8

1/124 kb

SBEIIa

4×

2A

1637

37.9

1368

31

1/58 kb

 

4×

2B

1641

37.8

1368

27

1/67 kb

SBEIIb

4×

2A

1909

36.8

600

15

1/61 kb

 

4×

2B

1972

36.8

1152

20

1/91 kb

  1. a 384 M2 plants were screened with LI-COR and 1152 M2 plants were screened using the polyacrylamide/ethidium bromide method.
  2. b All 768 M2 plants were screened with LI-COR.