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Table 2 PSSM and SIFT scores of WKS mutations.

From: A modified TILLING approach to detect induced mutations in tetraploid and hexaploid wheat

Gene

Domain

Line

Nucleotide Change

Amino Acid Change

PSSM

SIFT

Reaction to PST

WKS1

Kinase

T6-569

G 163 A

V 55 I

11.5

0.00

Susceptible

  

T6-89

G 508 A

D 170 N

10.4

0.46

Resistant

  

T6-312

G 585 A

G 199 R

19.7

0.00

Susceptible

  

T6-480-1

C 632 T

T 211 I

12.6

0.01

Susceptible

  

T6-138

G 914 A

R 305 H

13.6

0.01

Susceptible

 

START

T6-567

G 4437 A

D 477 N

12.3

0.00

Susceptible

WKS2

Kinase

T6-960

C 13 T

R 5 *

---a

---

Resistant

  

T6-480-2

G 72 A

W 24 *

---

---

Resistant

 

START

T6-826

G 2221 A

W 379 *

---

---

Resistant

  1. a PSSM and SIFT scores are not reported for mutations that cause premature stop codons
  2. Six WKS1 and three WKS2 mutants were scored as susceptible or resistant based on their reaction to Puccinia striiformis f. sp. tritici (PST) in Fu et al. [32]. In the nucleotide/amino acid change columns, the first letter indicates the wild type nucleotide/amino acid, the number its position from the start codon/methionine, and the last letter the mutant nucleotide/amino acid. High PSSM (>10) and low SIFT scores (<0.05) predict mutations with severe effects on protein function.