Skip to main content
Figure 2 | BMC Plant Biology

Figure 2

From: Isolation and functional characterization of cold-regulated promoters, by digitally identifying peach fruit cold-induced genes from a large EST dataset

Figure 2

Evaluation of the accuracy of the predicted expression patterns of selected genes by RT-PCR. (A) RT-PCR analysis of RNA expression of three cold-induced genes: Ppbec1, Ppxero2, and Pptha1 under different post-harvest conditions. These post-harvest conditions include: fruits processed in a packing plant (E1: non-ripe; no long term cold storage); packing followed by a shelf-life at 20°C for 2-6 days (E2: Ripe; no long term cold storage; juicy fruits); packing followed by cold storage at 4°C for 21 days (E3: non-ripe; long term cold storage) and packing followed by cold storage at 4°C for 21 days and shelf-life at 20°C for 2-6 days (E4: Ripe; long term cold storage; woolly fruits). The expression level of Pplox1 was analyzed as a control for genes that do not express preferentially in cold stored fruits (E3). Ppact7 was analyzed as a control for genes that do not significantly change expression levels between the four post-harvest conditions analyzed. The two arrows associated with each gel represent 500 bp (upper) and 300 bp (lower). The number of ESTs associated with each contig and library source is indicated. (B) Densitometry quantification of the expression level obtained by RT-PCR, the figure shows the bands intensities for each gene relative to Ppact7 intensity.

Back to article page