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Figure 5 | BMC Plant Biology

Figure 5

From: Isolation and functional characterization of cold-regulated promoters, by digitally identifying peach fruit cold-induced genes from a large EST dataset

Figure 5

Conserved heterologous regulation of the cold-inducible peach Ppbec1 and Ppxero2 promoters in transgenic Arabidopsis plants. The upper panel shows histochemical GUS staining of representative transgenic Arabidopsis lines carrying the Ppbec1 promoter-uidA fusion, Ppxero2 promoter-uidA fusion and Pptha1 promoter-uidA fusion. The lower panel shows the results of fluorometric GUS-assays of three independent Arabidopsis transgenic lines (L1, L2 and L3) containing the Ppbec1 promoter-uidA fusion, Ppxero2 promoter-uidA fusion or Pptha1 promoter-uidA fusion. Homozygous T3 plants were grown for 14 days in MS plates with 0.8% agar at 24°C (white bars) and then transfer to 4°C for 7 days (blacks bars). The asterisk above each bar represents those samples that have a statistically significant increase in GUS activity in the cold treated plants when compared to the untreated plants. Bars represent the mean ± standard deviation, n = 5. t-student * p < 0.01.

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