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Table 1 Concentrations of phenolic compounds and condensed tannins in non-inoculated and mycorrhizal silver birches

From: Phenolic compounds in ectomycorrhizal interaction of lignin modified silver birch

Leaves

 

Clone

Lines

  
 

T

A

23

44

65

Cinnamic acid derivatives

c

2.48 ± 0.44 a

2.12 ± 0.57 a

2.21 ± 0.37 a

2.30 ± 0.43 a

 

ECM

2.84 ± 0.71 a

2.35 ± 0.72 a

2.35 ± 0.41 a

2.50 ± 0.54 a

Flavonoids

c

26.14 ± 4.00 a

30.67 ± 8.64 a

28.85 ± 2.14 a

30.87 ± 8.68 a

 

ECM

27.07 ± 7.36 a

25.38 ± 9.78 a

25.80 ± 5.25 a

29.71 ± 5.80 a

Apigenin derivatives

c

0.20 ± 0.12 a

0.31 ± 0.10 a

0.31 ± 0.09 a

0.43 ± 0.25 a

 

ECM

0.40 ± 0.21 a

0.19 ± 0.11 a

0.24 ± 0.07 a

0.39 ± 0.12 a

Kaempherol derivatives

c

0.85 ± 0.13 a

0.71 ± 0.23 a

0.90 ± 0.19 a

0.68 ± 0.20 a

 

ECM

0.83 ± 0.14 a

0.73 ± 0.22 a

0.97 ± 0.13 a

0.69 ± 0.06 a

Myricetin derivatives

c

18.31 ± 3.58 a

22.81 ± 6.34 a

21.77 ± 1.75 a

21.66 ± 6.11 a

 

ECM

18.83 ± 5.04 a

18.40 ± 8.20 a

17.90 ± 4.30 a

20.17 ± 4.54 a

Quercetin derivatives

c

5.19 ± 1.34 a

6.34 ± 2.12 a

5.43 ± 1.45 a

7.05 ± 1.64 a

 

ECM

6.38 ± 1.52 a

5.52 ± 1.98 a

5.84 ± 1.18 a

7.20 ± 1.19 a

Condensed tannins

c

160.41 ± 24.97 a

142.87 ± 47.63 a

127.54 ± 34.23 a

154.82 ± 28.44 a

 

ECM

142.40 ± 68.98 a

106.7 ± 22.57 a

149.21 ± 24.76 a

142.32 ± 62.23 a

p-OH-cinnamic acid derivatives

c

3.21 ± 0.47 a

1.84 ± 0.49 b

2.46 ± 0,32 ab

2.71 ± 0.61 ab

 

ECM

3.10 ± 0.36 ac

2.22 ± 0.71 bc

2.42 ± 0.32 ac

2.42 ± 0.38 ab

Stems

 

Clone

Lines

  
 

T

A

23

44

65

Cinnamic acid derivatives

c

2.68 ± 0.68 a

1.37 ± 0.28 b

1.33 ± 0.25 b

1.65 ± 0.37 bc

 

ECM

2.33 ± 0.33 ac

1.46 ± 0.18 b

1.27 ± 0.30 b

1.18 ± 0.20 b

Flavonoids

c

14.33 ± 1.74 ab

11.43 ± 1.49 ab

10.23 ± 1.43 b

13.79 ± 2.81 ab

 

ECM

14.60 ± 2.12 a

12.38 ± 1.89 ab

10.77 ± 2.37 ab

11.18 ± 1.47 ab

Phenolic glycosides

c

16.86 ± 1.46 ab

19.48 ± 0.81 ab

18.18 ± 2.22 ab

22.67 ± 5.31 b

 

ECM

15.40 ± 2.74 a

20.61 ± 2.29 ab

20.61 ± 2.29 ab

17.37 ± 5.29 ab

Condensed tannins

c

108.57 ± 50.76 a

138.57 ± 13.52 a

137.43 ± 13.72 a

141.47 ± 16.70 a

 

ECM

144.82 ± 19.68 a

132.86 ± 11.23 a

131.90 ± 9.26 a

130.32 ± 12.55 a

Roots

 

Clone

Lines

  
 

T

A

23

44

65

Cinnamic acid derivatives

c

0.71 ± 0.33 a

0.17 ± 00.05 a

0.17 ± 0.10 a

0.37 ± 0.23 a

 

ECM

0.53 ± 0.30 a

0.19 ± 0.02 a

0.20 ± 0.13 a

0.24 ± 0.14 a

Flavonoids

c

11.39 ± 0.62 a

9.45 ± 1.83 a

8.41 ± 1.93 a

8.26 ± 2.26 a

 

ECM

11.24 ± 2.78 a

12.10 ± 2.55 a

7.88 ± 2.91 a

7.21 ± 1.25 a

Gallo/Ellagitannins

c

0.06 ± 0.01 a

0.32 ± 0.24 a

0.28 ± 0.09 a

0.12 ± 0.06 a

 

ECM

0.11 ± 0.06 a

0.36 ± 0.08 a

0.23 ± 0.12 a

0.15 ± 0.13 a

Condensed tannins

c

130.50 ± 22.43 a

113.87 ± 17.86 a

106.92 ± 27.75 a

112.24 ± 8.94 a

 

ECM

126.09 ± 6.80 a

116.24 ± 10.11 a

108.47 ± 5.62 a

100.46 ± 2.24 a

Condensed tannin precursors

c

35.07 ± 5.81 ab

31.44 ± 7.15 ab

28.60 ± 5.50 ab

28.50 ± 3.81 ab

 

ECM

37.20 ± 9.43 ac

41.92 ± 10.62 a

20.38 ± 6.70 b

25.44 ± 2.21 bc

  1. Concentrations (mg/DW g) of phenolic compounds and condensed tannins in the leaf, stem and root samples of silver birch clone A and PtCOMT-modified lines 23, 44 and 65 after 8 weeks in co-culture with P. involutus. Values are means ± standard deviations in the presence (ECM) or absence (c) of the fungus. Different letters following the values denote significant differences (P < 0.05) between the non-inoculated and mycorrhizal plants within the line/clone and between lines/clone within the fungal treatment according to the Kruskal-Wallis test combined with the Wilcoxon rank sum test with the Benjamini & Hochberg correction or the one-way or two-way Anova combined with Tukey's honestly significant difference test or with the two-sample t-test with the Benjamini & Hochberg correction. For statistical testing the leaf apigenin derivatives were square root, stem cinnamic acid derivatives log 10 and root condensed tannins square transformed. Number of replicates 4-7.