Table 1 List of primers utilized in the expression analysis
Gene | Tm | N°cycles | Primer forward | Primer reverse | Accession number |
|---|---|---|---|---|---|
MdLAX1 | 57 | 31 | 5'-AGTGCTATCAGTGCAAGC-3' | 5'-CCGGAGAATTTTTCTGACTG-3' | EF406263 |
MdLAX2 | 57 | 31 | 5'-CTACCACCACCACATCAC-3' | 5'-GGAAACACCACCAATTCAC-3' | EF406264 |
MdLAX3 | 57 | 31 | 5'-CATCACTAAGCTTCTCTCTC-3' | 5'-CAACACCAATCCACTCTTC-3' | EF406262 |
MdPIN1 | 71 | 33 | 5'-ATTTTACCACGGCGGGCAAGG-3' | 5'-CCTTCCCGCCATTGTTGTCCT-3' | EF406255/EF406256 |
MdPIN10 | 71 | 35 | 5'-CGGCGGACAGAATAATGCAGT-3' | 5'-GAAGATCCTTCCCGCCATTGG-3' | EF406260/EF406261 |
MdPIN4 | 71 | 37 | 5'-ACAATTCAACAGCAGCAGCA-3' | 5'-CGGTCCCACCAAAAACATGG-3' | EF406257 |
MdPIN7 | 71 | 32 | 5'-TTTGGGTTTTATCCCGCGCAGA-3' | 5'-CGGCGGCTGCCTGATTTTTGC-3' | EF406258/EF406259 |
MdIAA3 | 62 | 29 | 5'-GGTTGAGAATCATGAAGGGT-3' | 5'-AAAGCCCGAGCTCTATGTCT-3' | DQ848594 |
MdIAA7 | 64 | 30 | 5'-AATGCAAGAACAGGAGCTGA-3' | 5'-TCCAATCAGTCGAACAACCT-3' | DQ848598 |
MdAHS | 58 | 30 | 5'-CTTCGTTGGGTCAATTACG-3' | 5'-GGCAATAATCACTCCAAGG-3' | EF406254 |
MdMAX2 | 61 | 30 | 5'-ACATTGCGGAAGCTCTTC-3' | 5'-GCATCTTCGAATCGACAAC-3' | DY242015 |
MdARR6 | 61 | 30 | 5'-GCATCATCATCTCCCTCCTCT-3' | 5'-CAATCCATCGTGTGCAATCTC-3' | EF406267 |
MdARR3/4 | 61 | 30 | 5'-TGATCCGACGATTGCTCCATC-3' | 5'-CAGCGTTGGTCATTTTCAACC-3' | EF406265/EF406266 |
MdUBI | 61 | 28 | 5-CATCCCCCCAGACCAGCAGA-3 | 5-ACCACGGAGACGCAACACCAA-3 | DQ438989 |
r18S | 62 | 25 | 5-GTTACTTTTAGGACTCCGCC-3 | 5-TTCCTTTAAGTTTCAGCCTTG-3 |