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Table 4 Comparison of SCMV microarray and qRT-PCR results

From: Validation of candidate genes putatively associated with resistance to SCMV and MDMV in maize (Zea mays L.) by expression profiling

 

Fold of change

qRT-PCR performance

Target gene

Microarrays: four biological replications (average) ± SE

qRT: four biological replications (average) ± SE

Coefficients of determination (R) target/reference

PCR efficiencies target/reference

605018B03.x1

2.1 (± 0.16)

1.6 (± 0.18)

0.99/0.99

1.1/1.2

605018B04.x1

2.6 (± 0.15)

89.2 (± 14.82)

0.99/0.98

1.1/1.4

946126A02.y1

1.7 (± 0.18)

1.2 (± 0.18)

0.98/0.98

1.0/1.1

Zm06_09h07_R

1.6 (± 0.12)

1.4 (± 0.13)

0.99/0.99

1.1/1.1

946063C12.y1

2.0 (± 0.26)

2.7 (± 0.37)

0.97/0.99

1.1/1.3

za72g09.b50

1.4 (± 0.13)

2.7 (± 0.19)

0.94/0.99

3.6/1.1