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Figure 2 | BMC Plant Biology

Figure 2

From: The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

Figure 2

Phenotype of the exo mutant. A: Wild-type and exo (35 days after sowing). B: Wild-type and exo. Plants were grown in 0.5 × MS medium for 16 days under aseptic conditions. C: Wild-type, exo, and three plants of the segregating transgenic exo line # 4 (T2 generation) carrying the 35S::EXOga construct (29 days after sowing). Complemented exo plants (# 4-1 and # 4-3) showed detectable EXOga transcripts as demonstrated by northern-blot analysis. RNA samples from left to right as indicated above (exo, WT, 35S::EXOga plant #4-1, #4-2, and #4-3). Similar results were obtained for independent transgenic lines. D: Wild-type and exo (40 and 49 days after sowing). E: Wild-type, exo, and homozygous transgenic exo plant carrying the 35S::EXO:HA construct. Complemented exo plants showed detectable EXO:HA protein as demonstrated by Western analysis. Protein samples from left to right as indicated above (Col-0, exo, 35S::EXO:HA in exo). A monoclonal anti-HA antibody was used. Similar results were obtained for independent transgenic lines.

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