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Figure 7 | BMC Plant Biology

Figure 7

From: Phenotypic instability and epigenetic variability in a diploid potato of hybrid origin, Solanum ruiz-lealii

Figure 7

MSAP analysis of eight S. ruiz-lealii plants. A, representative MSAP profiles of two EcoRI/HpaII (H) and EcoRI/MspI (M) digest of DNA extracted from eight S. ruiz-lealii plants. The primer combinations used were E-AGA/HM-TCAA (left panel) and E-AGA/HM-TCCA (right panel). The arrows indicate positions of size markers. B, detail of the primer combination E-AGA/HM-TCCA. Arrows heads, fragments analyzed as "methylation sensitive polymorphism". Arrow, fragment analyzed as "methylation insensitive polymorphism". C, graphical interpretation of methylation sensitive fragments. The boxes represent the double-stranded recognition site (CCGG) of the HpaII-MspI isoschizomer. Black boxes indicate methylated cytosine. Fragments 1 and 2 epialleles present in plants with normal and intermediate flower phenotype. Fragments 3 and 4, specific epialleles of plants with abnormal flower phenotype. Fragments 5 and 6, methylated epialleles present in three plants with abnormal flower phenotype and in plant 6, with intermediate flower phenotype; and demethylated epialleles specific of plants with normal flower phenotype. aMethylation patterns not determined, because the absence of a MSAP fragment can result from either a full methylation of cytosines on both strands or the absence of the restriction sites.

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