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Table 1 Number of identified PR-10 sequences in nine birch species.

From: Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen

Species

Number of

sequenced

clones

Subfamily 01

Subfamily 02

Subfamily 03

Subfamily 04

Subfamily 05

Subfamily 06

Subfamily 07

Total

  

Seqs

Genes

Seqs

Genes

Seqs

Genes

Seqs

Genes

Seqs

Genes

Seqs

Genes

Seqs

Genes

Seqs

Genes

Subgenus Betulaster:

                 

B. nigra

155

10

6

4

3

7

4

2

1

2

1

-

-

-

-

25

15

Subgenus Neurobetula:

                 

B. chichibuensis

170

5

4

3

2

10

7

1

1

1

1

2

2

-

-

22

17

B. schmidtii

184

3

2

3

2

1

1

1

1

1

1

2

2

1

1

12

10

Subgenus Betulenta:

                 

B. lenta

106

3

2

3

2

4

4

1

1

2

1

-

-

1

1

14

11

Subgenus Betula:

                 

B. costata

103

9

8

3

2

5

5

1

1

2

1

-

-

-

-

20

17

B. pendula

102

5

3

2

2

5

4

2

1

2

1

-

-

-

-

16

11

B. plathyphylla

103

6

4

4

3

6

3

2

1

2

1

-

-

-

-

20

12

B. populifolia

106

4

4

4

2

5

3

2

1

2

1

-

-

-

-

17

11

B. pendula reference * 2

-

-

4

-

3

-

4

-

1

-

1

-

-

-

-

-

13

  1. The number of clones sequenced in both directions and the number of identified sequences and genes are shown per species.1 Subfamily 01 to 05 were previously identified [32], while subfamily 06 and 07 are new. Homology to mRNA sequences suggests that subfamily 01 and 02 are expressed in pollen.
  2. *1 Species were diploid (2n) as measured by flow cytometry. The identification of alleles of a single gene is based on the criterion of having > 98.5% sequence similarity, and by allowing maximally two alleles per gene.
  3. *2 Genes identified in B. pendula [32].
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BMC Plant Biology

ISSN: 1471-2229

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