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Figure 1 | BMC Plant Biology

Figure 1

From: Regulation of callose synthase activity in situ in alamethicin-permeabilized Arabidopsisand tobacco suspension cells

Figure 1

Alamethicin permeabilization of Col-0 cells. (A) Oxygen consumption in Col-0 and BY-2 cells after addition of 20 μg ml-1 alamethicin. Points represent the rate of oxygen consumption relative to the control rate prior to alamethicin addition. (B-F) Visualization of alamethicin permeabilization of Col-0 cells by Yo-Pro staining. Bright field microscopy images of untreated (B) and alamethicin-permeabilized (C) cells as well as fluorescent images showing Yo-Pro staining of untreated (D) and alamethicin-permeabilized (E-F) cells. (F) shows a close up of (E).

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