Figure 6

In situ hybridization pattern of At3g55560 in inflorescences. (A – F): anti-sense probe; (G, H): sense probe. (A) Transcripts of At3g55560 were detected in the outer cell layers of floral primordia and floral organ primordia, and were maintained in the medial region of the elongating carpel (B). (C, D) Expression specific to the placenta was observed prior to fusion of the septum. (E) Emerging ovule primordia showed specific expression, that was maintained most strongly in the outer cell layers of the chalaza as the ovules developed (F). Expression was maintained at low levels in the distal funiculus and chalaza as the integuments initiated. (G) The sense probe detected RNA in the megaspore mother cell and developing embryo sac, as well as in the tapetum and pollen at maturity (H), but not in the structures that hybridized to the anti-sense probe. Bar = 20 μm in A, B, and F (bottom); 5 μm in C, and G; 10 μm in D, E, and F (top); 40 μm in H. c, carpel; ch, chalaza; es, embryo sac; f, funiculus; fm, floral meristem; fp, flower primordium; im, inflorescence meristem; n, nucellus; oi, outer integument; op, ovule primordium; p, pollen; pl, placenta; se, septum; st, stamen.