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Figure 5 | BMC Plant Biology

Figure 5

From: A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis

Figure 5

Effect of proteasome inhibitor on stability of polyubiquitinated proteins. Polyubiquitination assays of crude FLAG-tagged E2s and E3s were carried out in the presence or absence of biotinylated ubiquitin and 20 μM MG132. A, FLAG-tagged recombinant proteins were detected by immunoblot analysis using anti-FLAG antibody. B, Polyubiquitination chain formed by each recombinant protein was detected by Alexa488-conjugated streptavidin.

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BMC Plant Biology

ISSN: 1471-2229

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