Figure 3From: Cloning of transgenic tobacco BY-2 cells; an efficient method to analyse and reduce high natural heterogeneity of transgene expressionA scheme of the BY-2 cloning procedure. (a) Mixture of transgenic and wild-type lines before plating onto solid media. (b) Cloned calli emerging on the feeder layer ~10 days after plating. (c) Cloned calli of a heterogeneous line observed with a fluorescence stereomicroscope. Scale bars: 100 μm for A, 1 mm for C.Back to article page