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Table 1 Frequencies of BY-2 calli and suspensions with homogeneous and heterogeneous GFP fluorescence

From: Cloning of transgenic tobacco BY-2 cells; an efficient method to analyse and reduce high natural heterogeneity of transgene expression

  

Primary suspensions

 

Secondary suspensions

GFP fluorescence in callus

Primary calli

Homogeneous

Heterogeneous

Secondary calli

Homogeneous

Heterogeneous

Homogeneous

39.3% ± 9.7%

29.2% ± 5.3%

70.8% ± 5.3%

93% ± 2.3%

46.3% ± 5.4%

53.7% ± 5.4%

Heterogeneous – mosaic

35.8% ± 13%

0%

100%

7% ± 2.3%

0%

100%

Heterogeneous – sectorial

24.9% ± 6.8%

0%

100%

0%

0%

100%

  

11.5% in total

88.5% in total

 

42.8% in total

57.2% in total

  1. Frequencies of homogeneous and heterogeneous calli and suspension cultures derived from these calli in primary lines obtained after transformation, and in secondary lines produced by cloning of primary heterogeneous (!) suspensions. Values represent means ± SD (n = 3); data are from three independent transformations; in every replication the number of evaluated lines was 60–80 for calli and ~20 for suspensions).
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BMC Plant Biology

ISSN: 1471-2229

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