Figure 6
Confocal microscopic analysis of FUL:GFP, SEP3:GFP and AG:GFP localisation in stage 12 pistils. (A) FUL:GFP localisation in a stage 12 pistil, with the highest signal in the valves and the replum, and lower signal in the valve margins and the basal half of the style. The image is composed of three separate, overlapping projections. Nuclei with FUL:GFP proteins are visible as bright green spots against the green autofluorescent background of the pistil. (B) SEP3:GFP localisation in the apical part of a stage 12 pistil with the highest signal in the valve margins and replum, and lower signal in the style and valves. (C) AG:GFP signal in all cell layers of the apical part of a stage 12 pistil. (D) SEP3:GFP localisation in the middle part of a stage 12 pistil with the highest signal in the valve margins and replum, and lower signal in the valves. (E) AG:GFP signal in all cell layers of the middle part of a stage 12 pistil. (F) Schematic cross sections of the FUL:GFP, SEP3:GFP and AG:GFP localisations in the ovary wall. (r) replum; (s) stigma; (st) style; (v) valve; (vm) valve margin. All scale bars 50 μm.