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Table 1 Characteristics and frequency of different types of SSRs identified in 1223 unigenes of tea

From: Identification, characterization and utilization of unigene derived microsatellite markers in tea (Camellia sinensis L.)

S. No.

SSRs details

No. primers designed

Primers recorded successful amplification

 

Repeat type

No.

Repeat motif units

No. of SSRs identified

Class I *

Class II **

  

1.

Di-nucleotides

61

(TA)n

5

5

 

3

2

   

(TC)n.(GA)n

51

48

3

47

29

   

(CA)n.(TG)n

5

5

 

2

1

2.

Tri-nucleotides

37

(TTC)n.(GAA)n

7

6

1

6

5

   

(TCC)n.(GGA)n

1

1

 

1

1

   

(TCG)n.(CGA)n

3

3

 

1

1

   

(CAT)n.(ATG)n

7

7

 

6

4

   

(TGG)n.(CCA)n

6

6

 

4

4

   

(CTG)n.(CAG)n

5

5

 

5

1

   

(CCG)n.(CGG)n

3

2

1

2

1

   

(TTA)n.(TAA)n

3

3

 

2

2

   

(CAA)n.(TTG)n

2

2

 

2

2

4.

Tetra-nucleotides

8

(TATG)n.(CATA)n

2

2

 

1

-

   

(TTTG)n.(CAAA)n

3

2

1

2

1

   

(TTTC)n. (GAAA)n

1

1

 

1

1

   

(TTGG)n.(CCAA)n

1

1

 

1

1

   

(ACTG)n.(CAGT)n

1

1

 

0

-

5.

Penta-nucleotides

9

(TTCCC)n.(GGGAA)n

1

1

 

-

-

   

(TTGTG)n.(CACAA)n

1

1

 

2

1

   

(GAGAA)n.(TTCTC)n

2

2

 

1

1

   

(TTTTA)n.(TAAAA)n

1

1

 

1

-

   

(CAAGC)n.(GCTTG)n

1

1

 

1

-

   

(GGAAA)n.(TTTCC)n

1

1

 

1

1

   

(CGCTG)n.(CTGCG)n

1

0

1

1

-

   

(TTCTC)n.(GTGAA)n

1

1

 

1

1

6.

Hexa-nucleotides

5

(GGGAGA)n.(TCTCCC)n

1

1

 

-

-

   

(CCCTAA)n.(TTAGGG)n

1

1

 

0

-

   

(TTTTTA)n.(TAAAAA)n

2

2

 

1

-

   

(CAAAAA)n.(TTTTTG)n

1

1

 

1

1

 

Total

120

 

120

113

7

96

61

  1. * SSR with repeat length ≥ 20 nucleotides; nts,
  2. ** SSR with repeat length ≥ 12 nts to ≤ 20 nts.