Table 4 Markers developed from BACend sequences of a positive BAC clone with both D-Scar0 and D-Scar7
From: Phenotypic and fine genetic characterization of the D locus controlling fruit acidity in peach
BACend marker | Primer sequence (5'-3') | Size* (bp) | Polymorphism | Annealing temp. (°C) | Enzyme |
|---|---|---|---|---|---|
F109-15-06 | F GTAGGATGAACTCAAAGGTG | 570 (J, F) | SNP | 52 | Tsp509I |
R GTTGGTAATGACACTGGCTA | |||||
R109-15-06 | F GTGGACTTCATCCCATCTAC | 540 (J, F) | SNP | 54 | HincII |
R GGTCCAGAAGATGATGCAC |