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Figure 3 | BMC Plant Biology

Figure 3

From: Molecular characterization of a rice mutator-phenotype derived from an incompatible cross-pollination reveals transgenerational mobilization of multiple transposable elements and extensive epigenetic instability

Figure 3

Examples of validation of mPing excision and insertion events by locus-specific PCR amplifications. PCR amplification products by using mPing-bracketing locus-specific primers on template DNA of the rice parent (Tong211), the mutant (Tong211-LP) and its 8 S1 progeny individual. (a) and (b) are excisions of mPing from the mutant Tong211-LP and/or some of its S1 progenies, while (c) and (d) are de novo insertions in the mutant Tong211-LP and/or some of its S1 progenies. Arrows refer to positions of the upper larger-sized bands and lower smaller-sized bands, the size difference between which is exactly 430 bp (the full-length of mPing), based on sequencing.

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