Table 3 PCR primers used in this work
Primer | Description | Sequence (5'-3') |
|---|---|---|
F1APT2 | external forward for 3' RACE | CTGGAGGCCGACATCAACTTCAATCTG |
F2APT2 | nested forward for 3' RACE | GGAGCTCSAAGTACCGCGGCGTCAC |
R1APT2 | external reverse for 5' RACE | TGGGTACAAACGCTGGTGCTGCTGAG |
R2APT2 | nested reverse for 5' RACE | AAGGCCGGCGATGATGTTGTCCCTCTTG |
AP2F4 | forward for "edge-to-edge" | CGGCCACCGCGCTCCCATGCCATA |
AP2 new R | reverse for "edge-to-edge" | CACACCCGTCGACCRCCGTCCAT |
F3APT2 | forward for DNA sequencing | GAYTGCGGGAAGCAGGTCTACTTG |
F4APT2 | forward for DNA sequencing | AGATGCTMCACCTGACGTCGAAAATGAG |
R5APT2 | reverse for DNA sequencing | CTTCAACTTCGCTGTCRAAMAGCCCAAGAT |
AP2*F2 | forward for qRT-PCR | GGCCGCTGGGAGGCAAGGATGG |
AP2Ta*R2 | reverse for qRT-PCR (genomes A, B and D) | GCCGCCCTGTCGTACGCCCTTG |
AP2Hch*R2 | reverse for qRT-PCR (genome H) | GAAGCGCCGCCCTATCGTAGGCTCT |
Actin F4 | forward for actin gene | ACCTTCAGTTGCCCAGCAAT |
Actin R4 | reverse for actin gene | CAGAGTCGAGCACAATACCAGTTG |