Figure 5

Autoradiography showing accumulation of [¹⁴C]-Suc in whole rosettes. A through H, Representative rosette and individual leaf from a wild type plant (A, B) and lines 1039 (C, D), 1070 (E, F), and 1133 (G, H), respectively. Black arrowheads point to a mature source leaf with ¹⁴C accumulation in the veins and clearing from the areoles, white arrowheads point to sink leaves with diffuse labeling throughout the lamina, and grey arrowheads point to transition leaves. I and J, A representative homozygous SALK_038124 (AtSUC2 -/-) plant showing modest ¹⁴C accumulation only in putative sink regions of sink and transition leaves. Dark areas of the autoradiographs labeled (*) are artifacts of the adhesive used to secure the lyophilized tissue to a cardboard support in preparation for exposure to the X-ray film. The leaf directed to the top of the figure in A, C, E, G and I is magnified in B, D, F, H, and J; Scale bars, 5 mm in A, C, E, G and I 1 mm in B, D, F, H, and J.