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Table 3 Exudation and retention of 14C in leaves after photosynthetic labeling

From: Effective carbon partitioning driven by exotic phloem-specific regulatory elements fused to the Arabidopsis thaliana AtSUC2 sucrose-proton symporter gene

Plant Line

Percent Cumulative Exudation

Soluble Residual

Insoluble Residual

 

0 hrs

2 hrs

4 hrs

9 hrs

20 hrs

  

WTa

0.0 ± 0.0b

20.8 ± 4.1

36.7 ± 6.5

56.4 ± 6.2

70.1 ± 6.2

19.4 ± 4.9

10.5 ± 2.2

SUC2p (1039)a

0.1 ± 0.2

16.6 ± 5.3

31.4 ± 8.7

52.8 ± 6.5

66.5 ± 5.7

21.4 ± 1.8

12.1 ± 5.3

CoYMVp (1070)

0.5 ± 0.4c

14.3 ± 3.1c

27.8 ± 6.1c

51.4 ± 4.6

67.9 ± 4.2

19.9 ± 1.3

12.2 ± 3.0

RolCp (1133)

0.0 ± 0.0

15.1 ± 4.4c

26.8 ± 7.1c

47.0 ± 4.2c

62.6 ± 2.6c

24.4 ± 1.6c

12.9 ± 1.9

  1. Percentage of 14C-photosynthate exuded from cut petioles and retained in the leaf 20 hours after photosynthetic labeling with 14CO2. Four mature leaves from each of four replicate plants were arranged in a 24 well microtiter plate and photosynthetically labeled for 20 minutes with 14CO2 in a sealed chamber three feet below a 400 W metal halide bulb. Exudation into 15 mM EDTA proceeded for 20 hours under ambient room light, and the EDTA solutions were changed and counted at the indicated time points.
  2. a [25]
  3. b Average ± Std. Dev., n = 4
  4. c Student's T-test, p ≤ 0.1 relative to WT.