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Table 1 Gene description, primer sequences and efficiency of the selected ESTs.

From: Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha

Gene identification/Gene description

E value/ID (%)

Primer sequence/Amplicon size

Amplification efficiency ± SD*

GeneBank Accession Number

Bbriz EF1

Elongation factor-1 alpha

4e-89/

166/179 (92%)

5'ACCCTCCTCTTGGTCGTTTT3'

5'AGCCCCTCATTTCTTCTTGG 3'

105 bp

0.87 ± 0.012

EZ000623

Bbriz EIF4A

Eukaryotic initiation factor 4A

4e-41/

88/100 (88%)

5'TAAGGTGGGGCTTGTTTTTG3'

5'ACAGCAGCACATACCACAGG3'

164 bp

0.94 ± 0.011

EZ000622

Bbriz GAPDH

glucose-6-phosphate dehydrogenase

2e-39/

86/121 (71%)

5'TGAATCTAGTCCATCCGCTTG3'

5'TCATCAGGCAGGGAAGCTA3'

124 bp

0.97 ± 0.009

GE617483

Bbriz GDP

glyceroldehyde-3-phosphate dehydrogenase

6e-22/

48/55(87%)

5'GGGCATTTTGGGTTATGTTG3'

5'TCCCCACTCGTTGTCATACC3'

146 bp

1.01 ± 0.009

EZ000624

Bbriz SUCOA

succinyl-CoA ligase (GDP-forming) beta-chain

e-107/

203/236 (86%)

5'CAGCAAGGGAGGAACCAGTA3'

5'TAGCGCAAGACCATCAACAA3'

130 bp

1.00 ± 0.008

GE617476

Bbriz TUB

putative tubulin alpha-5 chain

4e-51/

96/98 (97%)

5'ATGAAGGCGATGAAGGAGAA3'

5'GTACGCAATGGAATGGAACC3'

112 bp

1.01 ± 0.019

GE617477

Bbriz UBCE1

Ubiquitin-conjugating enzyme

Bbriz UBCE2

4e-31/

64/74 (86%)

5'GGTCTTGCTCTCCATCTGCT3'

5'CGGGCTGTCGTCTCATACTT3'

114 bp

5'ACCAGCACAAATCAAAGGA3'

5'GCCAAAGTATGAGACGACAGC3'

149 bp

0.92 ± 0.013

0.95 ± 0.015

GE617481

Bbriz UBI

ubiquitin/ribosomal protein

4e-06/

28/49 (57%)

5'GTCACTAAGCCATCGGTCGT3'

5'ACACGGACACAACCAGTTCA3'

112 bp

0.94 ± 0.020

GE617482

  1. *Amplification efficiency was calculated using the miner algorithm [21] and range from 0.5 (50%) to 1.5 (150%).